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Otolith Shape Analysis of Anchovy ( Engraulis encrasicolus) in the Black, Marmara and Aegean seas

Zeliha Erdoğan*, Hatice Torcu Koç, Cemal Turan


Otolith shape analysis is widely used for fish species identification and stock classification. The morphological structure of anchovy populations was examined with otolith characters using Truss network system. A total of 300 anchovy specimens were collected by commercial fishing vessels from six fishing areas, three from the Black Sea (Trabzon, Sinop, Istanbul), one from the Marmara Sea (Bandırma Gulf) and two from the Aegean Sea (Edremit Gulf, Izmir Gulf) between November 2001 and January 2002. Our data were subjected to univariate statistics of variance (ANOVA) and discriminant function analysis (DFA) by using SPSS 21 software version. Plotting DF1 and DF2 revealed that the Marmara stock was clearly separated from others in the discriminant space. The proportion of specimens correctly classified into their original group was the highest (70%) for the Marmara Sea samples. The most important discriminative otolith characters in distinguishing between the groups for the first and second discriminant functions were otolith width and otolith length. The Marmara Sea is the passageway between the Black Sea and Aegean Sea, and currents or water masses play an important role in its environmental conditions (e.g. temperature, salinity, food). Significant difference in the Marmara sea population may be attributed to geographical and environmental conditions suggesting separate management strategies for the resource sustainability.


Engraulis encrasicolus, anchovy, otolith shape analysis, stock discrimination

Volume 4, No 3, SUPPLEMENT I of SYMPOSIUM ARTICLES, pp 10-17, 2019

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  • Ayvazian, S.G., Bastow, T.P., Edmonds, J.S., How, J., Nowara, G.B. (2004). Stock structure of Australian herring (Arripis geogiana) in southwestern Australia. Fisheries Research, 67, 39–53.
  • Bailey, M.C., Heath, M.R. (2001). Spatial variability in the growth rate of blue whiting (Micromesistius poutassou) larvae at the shelf edge west of the UK. Fisheries Research, 50, 73–87.
  • Begg, G.A., Brown, R.W. (2000). Stock identification of haddock Melanogrammus aeglefinus on the Georges Bank based on otolith shape analysis. Transactions of the American Fisheries Society, 129, 935–945.
  • Bembo, D. G., Carvalho, G. R., Cingolani, N., Pitcher, T. J. (19969. Electrophoretic analysis of stock structure in Northern Mediterranean anchovies, Engraulis encrasicolus. ICES Journal of Marine Science, 53, 115–128.
  • Bouchenak-Khelladi,Y.,Durand, J.D., Magoulas, A., Borsa, P. (2008). Geographic structure of European anchovy: a nuclear-DNA study. Journal of Sea Research, 59: 269–278.
  • Burke, N., Brophy, D., King, P.A. (2008). Shape analysis of otolith annuli in Atlantic herring (Clupea harengus); a new method for tracking fish populations. Fisheries Research, 91, 133–143.
  • Cadrin, S. X. (2005). Morphometric landmarks. In: Cadrin SX, Friedland KD and Waldman JR (eds) Stock identification methods. Elsevier Academic Press., UK. 153–172.
  • Campana, S.E., Neilson, J.D. (1985). Microstructure of fish otoliths. Canadian Journal of Fisheries and Aquatic Sciences, 42, 1014–1032.
  • Campana, S.E., Casselman, J.M. (1993). Stock discrimination using otolith shape analysis. Canadian Journal of Fisheries and Aquatic Sciences, 50, 1062–1083.
  • Cardinale, M., Doering-Arjes, P., Kastowsky, M., Mosegaard, H. (2004). Effects of sex, stock, and environment on the shape of known-age Atlantic cod (Gadus morhua) otoliths. Canadian Journal of Fisheries and Aquatic Sciences, 61, 158– 167.
  • Fox, C.J., Folkvord, A., Geffen, A.J. (2003). Otolith micro-increment formation in herring Clupea harengus larvae in relation to growth rate. Marine Ecology Progress Series, 264, 83–94.
  • Gallego, A., Heath, M.R., Basfrod, D.J., Mac Kenzie, B.R. (1999). Variability in growth rates of larval haddock in the northern North Sea. Fisheries Oceanography. 8,77–92.
  • Gauldie, R.W., Nelson, D.G.A. (1990). Otolith growth in fishes. Comparative Biochemistry and Physiology A, 97A, 119–135.
  • Ihssen, P.E., Booke, H.E., Casselman, J.M., McGlade, J.M., Payne, N.R., Utter, F.M. (1981). “Stock Identification: materials and methods”. Canadian Journal of Fisheries and Aquatic Sciences, 38, 1838-1855.
  • Kristoffersen, J. B., Magoulas, A. (2008). Population structure of anchovy Engraulis encrasicolus L. in the Mediterranean Sea inferred from multiple methods. Fisheries Research, 91, 187–195. doi: 10.1016/j.fishres.2007.11.024
  • Legua, J., Plaza, G., Perez, D., Arkhipkin, A. (2013). Otolith shape analysis as a tool for stock identification of the southern blue whiting, Micromesistius australis. Latin American Journal of Aquatic Research, 41(3), 479-489.
  • Maclean, J.A., Evans, D.O. ( 1981). Stock concept, discreteness of fish stocks and fisheries management. Canadian Journal of Fisheries and Aquatic Sciences, 38, 1889-1898.
  • Magoulas, A., Tsimenides, N., Zouros, E. (1996). Mitochondrial DNA Phylogeny and the Reconstruction of the Population History os a Species: The Case of the European Anchovy (Engraulis encrasicolus). Molecular Biology and Evolution, 13 (1), 178-190.
  • Munk, P., Heath, M., Skaarup, B. (1991). Regional and seasonal differences in growth of larval North Sea herring (Clupea harengus L.) estimated by otolith microstructure analysis. Continental Shelf Research. 11, 641–654.
  • Nelson, C.S., Northcote, T.G., Hendy, C.H., (1989). Potential use of oxygen and carbon isotopic composition of otoliths to identify migratory and non-migratory stocks of the New Zealand common smelt: pilot study. N. Z. J. Marine and Freshwater Research, 23: 337-344.
  • Pawson, M.G., Jennings, S., (1996). A critique of methods for stock identification in marine capture fisheries. Fisheries Research, 25, 3–4.
  • Pla, C., Tudela, S., Garcia-Marin, J.L. (1996). Diversity and population structure of the European anchovy in the Mediterranean Sea (Abstract). Scientia Marina, 60(Supl.2): 285-286.
  • Russ, J.C. (1990). Computer-assisted Microscopy: The Measurement and Analysis of Images. New York: Plenum Press.
  • Sanz, N., Garcia-Marı´n, J. L., Vin˜as, J., Rolda´n, M., Pla, C. (2008). Spawning groups of European anchovy: population structure and management implications. ICES Journal of Marine Science, 65, 1635–1644.
  • Spanakis, E., Tsimenides, N., Zouros, E. (1989). Genetic differences populations of sardine, Sardina pilchardus, and anchovy, Engraulis encrasicolus, in the Aegean and Ionian seas. Journal of Fish Biology, 35: 417-437.
  • Strauss, R.E., Bookstein, F.L. (1982). The truss: body form reconstructions in morphometrics. Systematic Zoology, 31: 113-135.
  • Tudela, S. (1999). Morphological variability in a Mediterranean, genetically homogeneous population of the European anchovy, Engraulis encrasicolus. Fisheries Research, 42, 229–243.
  • Turan, C. (1999). A note on the examination of morphometric differentiation among fish populations: the truss system. Turkish Journal of Zoology., 23:259–264.
  • Tuset, V. M., Lozanı, I.J., Gonzalez, J.A., Pertusa, J.F., Garcia-Diaz, M.M. (2003). Shape indices to identify regional differences in otolith morphology of comber, Serranus cabrilla (L., 1758). Journal of Applied Ichthyology, 19, 88-93.
  • Tuset, V. M., Rosin, P.L., Lombarte, A. (2006). Sagittal otolith shape used in the identification of fishes of the genus Serranus. Fisheries Research, 81, 316-325.
  • Whitehead, P.J.P., Nelson, G.J., Wongratana, T. (1988). Clupeoid fishes of the world (Suborder Clupeoidei). An annotated and illustrated catalogue of the herrings, sardines, pilchards, sprats, shads, anchovies and wolf-herrings. FAO species catalogue, Vol. 7, Part 2 – Engraulididae, 579 pp.
  • Winans, G.A. (1984). Multivariate morphometric variability in Pacific salmon: technical demonstration. Canadian Journal of Fisheries and Aquatic Sciences, 41: 1150-1159.
  • Zengin, M., Saygın, S., Polat, N. (2015). Otolith shape analyses and dimensions of the anchovy Engraulis encrasicolus L. in the Black and Marmara Seas. Sains Malaysiana, 44(5), 657–662.