This Article Statistics
Viewed : 651 Downloaded : 419


Effects of Propolis on Serum Biochemical Parameters in Azaserine Treated Rats

Hasan Yıldız, Ümit Miçooğulları

DOI: 10.28978/nesciences.1142700


Azaserine (o-diazoacetyl-L-serine) is an antimetabolite obtained from streptomycetes cultures and is used experimentally to produce atypical acinar cell focus (AACF) in rat exocrine pancreas. Propolis is a resinous hive product with antioxidant, anticarcinogenic, antimicrobial and anti-inflammatory effects that bees collect from the plants around them. In this study, we aimed performed to investigate the effect of ethanol extract of propolis (EEP) on serum biochemical changes in azaserin induced pancreatic cancer in Wistar rats. For this purpose, male Wistar albino rats were randomly divided into 4 groups, 10 in each group. AzCt and AzProp group 2-week-old male rats were given azaserine (30 mg/kg bw) intraperitoneally (ip). Propolis (EEP) (oral 80 mg/lt) was added to the drinking water of Prop and AzProp group rats. For the first time in this study, the effects of propolis on rats with neoplastic structures formed by azaserine were investigated in terms of biochemical parameters (AST, ALP, ALT, BILD, BILT, CHOL, LDH, TRIG, UREA, TAS, TOS and OSI). According to this; AzProp group values were compared to AzCt group, respectively, AST, UREA, TOS and OSI, there is a statistically significant difference in terms of values. As a result, it was seen that the oxidative stress caused by azaserine-induced neoplastic structures decreased with the use of propolis and that propolis had a positive effect on the measured biochemical parameters.


Azaserine, propolis, serum, oxidative stress

Download full text   |   How to Cite   |   Download XML Files

  • Baggott, J.E., Gorman, G.S. & Tamura, T., (2007). 13C enrichment of carbons 2 and 8 of purine by folate-dependent reactions after [13C]formate and [2-13C] glycine dosing in adults humans. Metabolism Clinical and Experimental, (56): 708-715. doi:10.1016/j.metabol.2006.12.020
  • Boyle, P., & Levin, B., (2008). WHO, World Cancer Report, Lyon, France: International Agency for Research on Cancer, 1-105.
  • Catchpole, O., Mitchell, K. Bloor, S., Davis, P., & Suddes, A., (2015). Antiproliferative activity of New Zealand propolis and phenolic compounds vs. human colorectal adenocarcinoma cells. Fitoterapia (106), 167–174. https://doi.org/10.1016/j.fitote.2015.09.004.
  • Çetin E., Kanbur M., Silici S., & Eraslan G., (2010). Propetamphos-induced changes in haematological and biochemical parameters of female rats: Protective role of propolis. Food and Chemical Toxicology, 48(7), 1806-1810. https://doi.org/10.1016/j.fct.2010.04.010
  • Erel, O., (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation, Clinical Biochemistry, 37(4), 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015.
  • Erel, O., (2005) A new automated colorimetric method for measuring total oxidant status, Clin Biochem, 38(12), 1103-1111. https://doi.org/10.1016/j.clinbiochem.2005.08.008.
  • Iqbal, M., Fan, T.P., Watson, D., Alenezi, S., Saleh, K. & Sahlan, M. (2019). Preliminary studies: The potential anti-angiogenic activities of two Sulawesi Island (Indonesia) propolis and their chemical characterization. Heliyon, 19,5(7),e01978. https://doi.org/10.1016/j.heliyon.2019.e01978.
  • Longnecker D.S. & Curphey T.J. (1975). Adenocarcinoma of the pancreas in azaserine-treated rats. Cancer Res, 35(8), 2249-58.
  • Mani F, Damasceno HC, Novelli EL, Martins, EA & Sforcin JM. ( 2006). Propolis: Effect of different concentrations, extracts and intake period on seric biochemical variables. J Ethnopharmacol;105:95‑8. doi: 10.1016/j.jep.2005.10.011.
  • Patel, S., (2016). Emerging Adjuvant Therapy for Cancer: Propolis and its Constituents, Journal of Dietary Supplements, 13(3), 245–268. https://doi.org/10.3109/19390211.2015.1008614.
  • Popova, M., Giannopoulou, E., Skalicka-Wózniak, K., Graikou, K., Widelski, J., Bankova, V., & Antosiewicz, B., (2017). Characterization and biological evaluation of propolis from Poland. Molecules 22(7), 1159. https://doi.org/10.3390/molecules22071159.
  • Prajapati A.S. Raval S.K., Sinha S., Varia T.N., & Mashiyava P.H., (2015). Effect of Phyllanthus amarus on serum biochemical changes in azaserine induced pancreatic cancer in wistar rats, Veterinary World, 8(8), 937-940. https://doi.org/10.14202/vetworld.2015.937-940.
  • Przybyłek, I., & Karpinski, T.M. (2019). Antibacterial properties of propolis. Molecules, 24(11), 2047. https://doi.org/10.3390/molecules24112047.
  • Revathi, R., Murugesan, M. & Manju, V. (2012) Protection against azaserine induced pancreatic cancer in rats by Phyllanthus amarus: A preliminary study. J. Biochem. Technol., 3(4): 331-335.
  • Satué, K., Miguel-Pastor, L., Chicharro, D. & Gardón, J.C. (2022). Hepatic Enzyme Profile in Horses. Animals, 12, 861. https://doi.org/10.3390/ani12070861.
  • Sforcina J.M., & Bankovab V. (2011). Propolis: Is there a potential for the development of new drugs? Journal of Ethnopharmacology 133(2), 253–260. https://doi.org/10.1016/j.jep.2010.10.032.
  • Shittu O.K., Lawall B., Alozieuwal B.U., Haruna G.M., Abubakarl A.N. & Berinyuy E.B,. (2015). Alteration in biochemical indices following chronic administration of methanolic extract of Nigeria bee propolis in Wistar rats. Asian Pac J Trop Dis 2015; 5(8): 654-657 doi: 10.1016/S2222-1808(15)60907-0.
  • Siegel R.L., Miller K.D. & Jemal A. (2017). Cancer Statistics. Cancer J Clin, 67(1), 7-30. https://doi.org/10.3322/caac.21387.
  • Stojanovi´c, S., Najman, S.J., Bogdanova-Popov, B., & Najman, S.S. (2020). Propolis: Chemical composition, biological and pharmacological activity—A Review. Acta Med. Median. 59(2), 108–113. https://doi.org/10.5633/amm.2020.0215.
  • Zabaiou, N., Fouache, A., Trousson, A., Buñay-Noboa, J., Marceau, G., Sapin, & Lobaccaro, J.M.A. (2019). Ethanolic extract of Algerian propolis decreases androgen receptor transcriptional activity in cultured LNCaP cells. J. Steroid Biochem. (189), 108–115. https://doi.org/10.1016/j.jsbmb.2019.02.016.