This Article Statistics
Viewed : 1814 Downloaded : 1397


Assessment of DNA Damage by Comet Assay in Trachinotus ovatus Cells from Mersin Bay in the Northeastern Mediterranean

Funda Turan* and Ayşegül Ergenler


The aim of this study is to get database to elucidate the quantity of DNA damage in Trachinotus ovatus from Mersin Bay in the North-eastern Mediterranean. Primarily, we have used the single-cell gel electrophoresis, commonly known as Comet assay to detect the strand breaks in DNA. This technique was performed on liver and gill tissues of T. ovatus captured from the Mersin Bay. The single cell gel electrophoresis was executed under alkaline conditions. The slides were neutralized with ice cold 0.4 M Tris buffer (pH 7.5) and stained with 80 ml ethidium bromide (20 mg/ml) and imaged with attachment of Leica fluorescent microscope integrated CC camera. DNA damage levels were determined from 100 cells for each sample. As a result of the COMET analysis; tail length, tail ıntensity and tail migration were 39.718±6.826 µm, 32.752±9.281% and 30.089±9.930 in gill tissues while they were 29.440±9.889 µm, 30.010±6.222 % and 19.119±5.025 in liver tissues respectively. it can be concluded that COMET analysis in T. ovatus from Mersin Bay can be a useful tool for screening genotoxic pollutants in the recipient environment.


Comet assay, DNA damage, Trachinotus ovatus

Volume 4, No 3, SUPPLEMENT I of SYMPOSIUM ARTICLES, pp 25-31, 2019

Download full text   |   How to Cite   |   Download XML Files

  • Ahmad, I., & Ahmad, M. (2016). Fresh water fish, Channa punctatus, as a model for pendimethalin genotoxicity testing: A new approach toward aquatic environmental contaminants. Environmental toxicology, 31(11), 1520-1529.
  • Ahmed, M. K., Kundu, G. K., Al-Mamun, M. H., Sarkar, S. K., Akter, M. S., & Khan, M. S. (2013). Chromium (VI) induced acute toxicity and genotoxicity in freshwater stinging catfish, Heteropneustes fossilis. Ecotoxicology and environmental safety, 92, 64-70.
  • Akter, R., Hasan, S. R., Siddiqua, S. A., Majumder, M. M., Hossain, M. M., Alam, M. A.& Ghani, A. (2008). Evaluation of Analgesic and Antioxidant Potential of the Leaves of Curcuma alismatifolia Gagnep. Stamford Journal of Pharmaceutical Sciences, 1(1), 3-9.
  • Ameur, W. B., de Lapuente, J., El Megdiche, Y., Barhoumi, B., Trabelsi, S., Camps, L., ... & Borràs, M. (2012). Oxidative stress, genotoxicity and histopathology biomarker responses in mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) liver from Bizerte Lagoon (Tunisia). Marine pollution bulletin, 64(2), 241-251.
  • Brenerman, B. M., Illuzzi, J. L., & Wilson III, D. M. (2014). Base excision repair capacity in informing healthspan. Carcinogenesis, 35(12), 2643-2652.
  • Collins, A. R., Oscoz, A. A., Brunborg, G., Gaivao, I., Giovannelli, L., Kruszewski, M.& Štětina, R. (2008). The comet assay: topical issues. Mutagenesis, 23(3), 143-151.
  • Diekmann, M., Waldmann, P., Schnurstein, A., Grummt, T., Braunbeck, T., & Nagel, R. (2004). On the relevance of genotoxicity for fish populations II: genotoxic effects in zebrafish (Danio rerio) exposed to 4-nitroquinoline-1-oxide in a complete life-cycle test. Aquatic toxicology, 68(1), 27-37.
  • Fatima, M., Tan, R., Halliday, G. M., & Kril, J. J. (2015). Spread of pathology in amyotrophic lateral sclerosis: assessment of phosphorylated TDP-43 along axonal pathways. Acta neuropathologica communications, 3(1), 47.
  • Frenzili, G., Nigro, M., & Lyons, B. P. (2009). A review: Use of the comet assay in aquatic environmental impact assessments. Mutatation Research, 681, 80-92.
  • Jha, A. N. (2008). Ecotoxicological applications and significance of the comet assay. Mutagenesis, 23(3), 207-221.
  • Klobučar, G. I., Štambuk, A., Pavlica, M., Perić, M. S., Hackenberger, B. K., & Hylland, K. (2010). Genotoxicity monitoring of freshwater environments using caged carp (Cyprinus carpio). Ecotoxicology, 19(1), 77.
  • Końca, K., Lankoff, A., Banasik, A., Lisowska, H., Kuszewski, T., Góźdź, S., & Wojcik, A. (2003). A cross-platform public domain PC image-analysis program for the comet assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 534(1-2), 15-20.
  • Mayer, C., Popanda, O., Zelezny, O., von Brevern, M. C., Bach, A., Bartsch, H., & Schmezer, P. (2002). DNA repair capacity after γ-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes. DNA repair, 1(3), 237-250.
  • McArt, D. G., McKerr, G., Howard, C. V., Saetzler, K., & Wasson, G. R. (2009). Modelling the comet assay. Biochemical Society Transactions, 37 (4), 914–917.
  • Mitchelmore, C. L., Birmelin, C., Chipman, J. K., & Livingstone, D. R. (1998). Evidence for cytochrome P-450 catalysis and free radical involvement in the production of DNA strand breaks by benzo [a] pyrene and nitroaromatics in mussel (Mytilus edulis L.) digestive gland cells. Aquatic Toxicology, 41(3), 193-212.
  • Møller, P. (2018). The comet assay: ready for 30 more years. Mutagenesis, 33(1), 1-7.
  • Ostling, 6., & Johanson, K. J. (1984). Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochemical and biophysical research communications, 123(1), 291-298.
  • Rajaguru, P., Suba, S., Palanivel, M., & Kalaiselvi, K. (2003). Genotoxicity of a polluted river system measured using the alkaline comet assay on fish and earthworm tissues. Environmental and Molecular Mutagenesis, 41(2), 85-91.
  • Rydberg, B., & Johanson, K. J. (1975). Radiation-induced DNA strand breaks and their rejoining in crypt and villous cells of the small intestine of the mouse. Radiation research, 64(2), 281-292.
  • Shaposhnikov, S., Azqueta, A., Henriksson, S., Meier, S., Gaivão, I., Huskisson, N. H.,& Collins, A. R. (2010). Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation. Toxicology letters, 195(1), 31-34.
  • Schmeiser, H. H., Muehlbauer, K. R., Mier, W., Baranski, A. C., Neels, O., Dimitrakopoulou-Strauss, A. & Kopka, K. (2019). DNA damage in human whole blood caused by radiopharmaceuticals evaluated by the comet assay. Mutagenesis, 34(3), 239–244.
  • Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H. & Sasaki, Y. F. (2000). Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and molecular mutagenesis, 35(3), 206-221.
  • Udroiu, I. (2006). Feasibility of conducting the micronucleus test in circulating erythrocytes from different mammalian species: an anatomical perspective. Environmental and molecular mutagenesis, 47(9), 643-646.