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Optimization of graphite-mineral oil ratio with response surface methodology in glucose oxidase based carbon paste electrode design

Esra Yağız, Gul Ozyilmaz*, Ali Tuncay Ozyilmaz

DOI: 10.28978/nesciences.1098655

Abstract

In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase (GOD) on carbon paste electrode (CPE) which was coated with polyaniline (PANI) by cyclic voltammetry technique. Since the current values to be measured depending on the glucose concentration will be affected by the graphite:mineral oil composition of the electrode, this parametres were optimized by response surface methodology (RSM). For this, State Ease Design Expert 8.0.7.1. (Serial Number: 0021-6578) software programme was used applying Optimal Design. By using data obtained, Design Expert programme suggested quadratic model to predict current values in terms of working parameters. In study, experimentally measured current values and predicted values by model were considerably found compatable and suitability of model was supported by ANOVA test.

Keywords

Carbon paste electrode, Response Surface Methodology, Glucose Oxidase, biosensor, polyaniline

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