- Esra Yağız
University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry, 31040, Hatay, Turkey
- Gül Ozyilmaz
University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry, 31040, Hatay, Turkey
gozyilmaz@gmail.com - Ali Tuncay Ozyilmaz
University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry, 31040, Hatay, Turkey
Volume 7 - No: 1
Optimization of Graphite-Mineral Oil Ratio With Response Surface Methodology in Glucose Oxidase Based Carbon Paste Electrode Design
DOI: 10.28978/nesciences.1098655
Keywords: Carbon paste electrode, Response Surface Methodology, Glucose Oxidase, biosensor, polyaniline
Abstract
In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase (GOD) on carbon paste electrode (CPE) which was coated with polyaniline (PANI) by cyclic voltammetry technique. Since the current values to be measured depending on the glucose concentration will be affected by the graphite:mineral oil composition of the electrode, this parametres were optimized by response surface methodology (RSM). For this, State Ease Design Expert 8.0.7.1. (Serial Number: 0021-6578) software software was used applying Optimal Design. By using data obtained, Design Expert software suggested quadratic model to predict current values in terms of working parameters. In study, experimentally measured current values and predicted values by model were considerably found compatable and suitability of model was supported by ANOVA test.