Abstract

Olea europaea L., frequently recognized for its traditional medicinal uses, contains bioactive compounds with antioxidant, anti-inflammatory, and metabolic regulatory properties. While these compounds have been widely investigated for their properties on human well-being, their consequences on aquatic species remain less discovered. The aim is to assess the effects of seven olive leaf metabolites caffeic acid, oleuropein, corosolic acid, moronic acid, lupeol, cycloartenol, and betulinic acid on liver metabolism in Nile tilapia (Oreochromis niloticus). The metabolites were incorporated into the fish food at three absorptions: 1g/100g, 3g/100g, and 5g/100g feed, and the fish were fed twice daily for 96 hours. Biochemical examination of fish serum revealed significantly improved Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and glucose levels associated with the control group. Particularly, corosolic acid and moronic acid encouraged the greatest noticeable development in liver enzyme activities, signifying potential disruptions in liver metabolism and function. These consequences indicate that bioactive compounds in olive leaves can influence liver physiology in aquatic species, emphasizing their potential effect on fish health. The research delivers valuable insights into the metabolic effects of these complexes, with implications for aquaculture nutrition approaches. Further investigation are essential to assess their long-term security and effectiveness.