Abstract

A study was done to test the effect of different factors on the multiplication and growth of fig plants in vitro. They were explants excised from two different cultivars wazziri and mission cultivar locally named (Bathinjani) and inoculated on two types of media murashiage and skooge 1962 (MS) and woody plant media (WPM) results were recorded after 6 weeks, where MS media gave a highest shoot number, leaf number and shoot length (1.50, 3.10, and 2.57) respectively, Bathinjani cultivar gave maximum means of shoots and leaves number (1.5 and 2.73)respectively, Bathinjani proliferated on MS media gave highest value of same characteristic mention above (1.67, 3.20 and 2.97) respectively, explants of the best cultivar were transferred into the most efficient medium. An explant 1cm length of the Bathinjani cultivar was cultured in MS medium containing TDZ at levels (0, 0.5, 1, 1.5, 2, 2.5 and 3) mg.-l to study its effect on shoot multiplication and growth the result showed the highest shoot number and its length was 7.20 shoot, 2.33cm respectively at 2mg.l -1 with a significant increase compared with rest treatments followed by concentration at 1.5 mg.l-1 also gave significant increasing of shoot number 5.22 so the concentration at 2.5 achieved high value of shoot length was 2.20cm, then plantlet subculture on MS media of rooting stage which contain half strength MS with adding Auxines IAA and IBA at (0, 0.25, 0.5 and 1) mg.l-1 for both, the data appeared IBA and IAA at 0.5 mg.l-1 gave highest value of root number and rooted percentage reached to (2.77, 2.38) and (99.33, 96.08 ) % respectively compared with 0.0 and 1 mg.l-1 gave lowest value for same characteristics.