Abstract

Glucose Oxidase (GOD) oxidizes glucose to -gluconolactone in presence of moleculer oxygen by forming hydrogen peroxide. As a result of the catalysed reaction, GOD is widely used in cases where glucose or molecular oxygen should be removed to extend the shelf life of foods or used in the production of controlled hydrogen peroxide or gluconic acid. One of the most important application areas of GOD is the construction of the glucose biosensors. There are several studies about GOD purification, immobilization, industrial and analytical applications, so, fast and sensitive determination of GOD activity is essential for these studies. In this study, GOD activity determination methods were reviewed mainly four approaches: determination of decrease in glucose or oxygen concentration and determination of increase in hydrogen peroxide or gluconic acid levels.