Abstract

This research examines genetic damage induced by metal pollution, using the golden grey mullet (L. aurata) as a bioindicator in the coastal region where Sarıseki Stream meet with Iskenderun Bay. The COMET assay and Micronucleus (MN) test were used to collect samples of L. aurata and coastal saltwater once a season for a year from both the sampling location and a reference site. The analysis showed that the concentrations of metals in water samples from both areas were below national and international standards. However, seawater from the sampling site had higher levels of chromium (Cr), lead (Pb), iron (Fe), and zinc (Zn) than the reference site in all seasons and on an annual basis. Notably, L. aurata from the sampling site exhibited a higher incidence of DNA damage and nuclear anomalies compared to specimens from the reference site, with significantly elevated micronucleus (MNi) values detected in the erythrocytes of L. aurata collected from the affected site throughout all seasons (P < 0.01). This study elucidates the genotoxic effects of pollution on L. aurata in this specific coastal area of the Northeastern Mediterranean. The results highlight the need for more study, including in vivo and in situ investigations, to assess the impacts of metals and other contaminants more thoroughly.