Volume 6 - No: 2
Extraction and Characterization of Chitin and Chitosan from Invasive Alien Swimming Crab Charybdis longicollis
- Servet Ahmet Doğdu
Molecular Ecology and Fisheries Laboratory, Marine Science Department, Faculty of Marine Science and Technology, Iskenderun Technical University, 31220 Iskenderun, Hatay, Turkey.
servetdogdu@hotmail.com
- Cemal Turan
Molecular Ecology and Fisheries Laboratory, Marine Science Department, Faculty of Marine Science and Technology, Iskenderun Technical University, 31220 Iskenderun, Hatay, Turkey.
- Tolga Depci
The Graduate School of Engineering & Sciences, Iskenderun Technical University, Iskenderun, Hatay, Turkey
Keywords: Charybdis longicollis, swimming crab, chitin, chitosan, scaffold, biomaterial
Abstract
Chitosan is a biocompatible, biodegradable, non-toxic, antibacterial, antioxidant, and antifungal natural polymer. In this study, chitin and chitosan were chemically isolated from the exoskeleton of invasive swimming crab Charybdis longicollis. In order to obtain the chitin, demineralization, deproteinization, and decolourization steps were applied to the samples. Chitosan was prepared from the isolated chitin by deacetylation at high temperatures. The chemical composition of chitin from C. longicollis was characterized by XRD and FTIR analysis. The yield of chitin extraction from dry crab shells was 25.78 %. The yield of chitosan produced from extracted chitin was 80.23 %. The experimental analyses revealed that the obtained chitin and chitosan could be used as biomaterial.